Structural and biochemical insights into the substrate selectivity and catalysis of hexachlorobenzene monooxygenase

Hexachlorobenzene (HCB), as one of the persistent organic pollutants (POPs) and possible human carcinogens, particularly resistant to biodegradation. In this study, HcbA1A3, a different flavin-N5-peroxide using enzymes and the only known naturally aerobic dechlorinase HCB, the biochemical marked.

Its clear preference for HCB in the binding affinity of sets that can only oxidize HcbA1 HCB than less chlorinated benzene as pentachlorobenzene and tetrachlorobenzenes. In addition, the crystal structure and complex HcbA1 with FMN resolved, revealing HcbA1 as new members of the bacterial luciferase-like family. Compared with a close homolog, the substrate binding pocket is much smaller than HcbA1 show limited space requirements for catalysis.

In the midst of an active, Tyr362 and Asp315 necessary in maintaining a normal conformation HcbA1, while Arg311, Arg314, Phe10, Val59 and Met12 is important for substrate affinity. They should have a place in the productive orientation HCB through multiple interactions. His17, with close contact with the oxidation locations HCB, possibly targeting the improvement of chlorine atoms and stabilizing the reaction intermediates.

Enzymatic characteristics and crystal structures reported here provide new insights into the substrate specificity and catalytic mechanism HcbA1, which opens the way for rational engineering and applications in bioremediation of polluted HCB environments.IMPORTANCE As an endocrine disruptor and possible carcinogen to humans, hexachlorobenzene (HCB) is resistant to biodegradation, largely due to difficulties in dechlorination.

Lack of knowledge to dechlorinases HCB limit their application in bioremediation. More recently, a HcbA1A3 monooxygenases reported HCB, which is the only known naturally aerobic HCB dechlorinase so far. Here we report the biochemical and structural characterization, which provides new insight into the substrate selectivity and catalytic mechanism. The study also improve our understanding of dechlorinases HCB and flavin-N5-peroxide using enzymes.

Ensuring the biochemical function of pectin methylesterase important in microbial gut Bacteroides thetaiotaomicron

Pectin is a major food source of nutrients for the human intestinal microbiota (HGM). Bacteroides thetaiotaomicron protruding gut microbes was recently shown to encode a founding member of (BT1017) of a new family of pectin methylesterases (PMEs) important for the metabolism of pectin complex rhamnogalacturonan-II (RG-II).

However, the biochemical and structural knowledge of this family less. Here, we show that the BT1017 is very important for the metabolism of a ΔBT1017oligoB oligosaccharides derived RG-II produced by BT1017 deletion mutant (ΔBT1017) during growth on carbohydrate extract of apple juice.

ΔBT1017oligoB structural analysis using a combination of enzymatic, mass spectrometry and nuclear magnetic resonance approach to reveal that it was a lady-oligosaccharides bi-methylated GlcA-β1,4- (2-O-Me-Xyl-α1,3) -Fuc- α1,4- (gALA-β1,3) -Rha-α1,3-Fire-β1,2- (Araf-α1,3) – (gala-α1,4) -GalA component containing RG-II backbones and side chains. We show that the catalytic module BT1017 adopt an alpha / beta (α / β) hydrolase fold, which consists of 10 twisted β-sheet strand middle sandwiched by multiple α-helices. It is a new fold for PMEs, which is dominated by left-handed β-helix proteins.

Bioinformatics analysis revealed that the family is dominated by a sequence of genera protruding from HGM, including Bacteroides and Prevotella Our results not only highlight the important role played by this family of enzymes in the metabolism of pectin but give new insights into the molecular basis of adaptation B. thetaiotaomicron to gut human.

Cellular and biochemical mechanisms of meroterpenoid Oncocalyxone antileukemic A

Oncocalyxone A, 1,4-benzokuinon derived from Cordia oncocalyx, showed anti-inflammatory, antimicrobial and anti-diabetic. The purpose of this study was to (1) examine the cytotoxic action oncocalyxone A on normal and tumor cell lines of human and (2) determine the mechanistic action effects on leukemia cells using the technique of the underlying cellular and molecular.

Antiproliferative studies on cancer cell lines, cells of peripheral blood mononuclear cells, and human erythrocytes done using colorimetric tests. To understand cytotoxicity, an assessment carried out by the HL-60 leukemia cells (8, 16.5, or 33 M) after 24 h incubation by using light and fluorescence microscopy, trypan blue, cytometry, Comet assay, western blot caspases and poly-ADP- flow ribose polymerase (PARP), and the effect on topoisomerase I and II. A Oncocalyxone exhibited cytotoxic action in HL-60 cells and dividing leukocytes, but minimal hemolytic action on erythrocytes.

The mechanistic investigation showed a decrease in cell viability, loss of membrane integrity, cells shrink, chromatin condensation, blebbings, phosphatidylserine externalization, caspase activation, PARP cleavage, mitochondrial depolarization and DNA damage. Pre-treatment with N-acetylcysteine ​​4 mM significantly reduced DNA damage and prevent the loss of membrane integrity. Oncocalyxone A free radical activity depends shown antileukemic through apoptosis and DNA damage induced in HL-60 cells.

Oncocalyxone A has a chemical structural simplicity allows it to be a cost-effective alternative. These properties justify further improvements to improve the activity and selectivity and pharmaceutical formulation development. Abbreviations Acridine orange, AO; ANOVA, analysis of variance; BSA, bovine serum albumin; IN, Damage Index; DMSO, Dimethylsulfoxide; EC50, the effective concentration of 50%; EDTA, ethylenediamine tetraacetic acid; EB, ethidium bromide; HCT-116, colon carcinoma lines; HL-60, promyelocytic leukemia line; IC50, 50% inhibitory concentration; MTT, 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide; OVCAR-8 ovarian carcinoma line; NAC, N-acetylcysteine, PBMC, peripheral blood mononuclear cells; PBS, phosphate-buffered saline; PI, propidium iodide; PARP, poly-ADP-ribose polymerase; RPMI-1640 media Roswell Park Memorial Institute; SF-295 glioblastoma line; ROS, reactive oxygen species; 7-AAD, 7-amino-aktinomisin D; H2-DCF-DA, 7′-dichlorodihydrofluorescein diacetate.

Biochemistry and in silico identification of the active site and catalytic mechanisms of circadian HESPERIN deadenylase

24h a molecular clock based on the stability of the transcript rhythmic disclosed. Shortening of the poly (A) tail of the mRNA is often the first step and the rate-limiting determine the life span of mRNA and catalyzed by deadenylases.

Here, we determine the catalytic site Hesperin, a circadian deadenylase recently described in plants, using a modified site-directed mutagenesis protocols and custom vectors, pATHRA. To explore the catalytic efficiency of AtHESPERIN we examined the effect of AMP and neomycin, and used molecular modeling simulations to propose a catalytic mechanism.

Collectively, biochemistry and classify AtHESPERIN silico results in EEP deadenylase superfamily and contribute to the understanding of the complex mechanisms of circadian mRNA turnover.

 

 

ALOX5 Antibody
1-CSB-PA001624GA01HU	Cusabio	Ask for price Ask for price	50ul 150ul
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IF

ALOX5 Antibody
1-CSB-PA001624LA01HU	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ALOX5 Antibody
1-CSB-PA008474	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

ALOX5 Antibody
1-CSB-PA787659	Cusabio	Ask for price Ask for price	50ul 100ul
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:500-1:2000, IHC:1:25-1:100

ALOX5 Antibody
1-CSB-PA043268	Cusabio	Ask for price Ask for price	50ul 100ul
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:500-1:5000, IHC:1:50-1:150

ALOX5 Antibody
E035564	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

ALOX5 Antibody
CAC08226-100ug	Biomatik Corporation	100ug	EUR 314

ALOX5 Antibody
CAC08226-50ug	Biomatik Corporation	50ug	EUR 199.2

ALOX5 Antibody
E92158	EnoGene	100ul	EUR 255
Description: Available in various conjugation types.

ALOX5 Antibody
E315047	EnoGene	100ug/200ul	EUR 295
Description: Available in various conjugation types.

ALOX5 Antibody
E19-6881	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

ALOX5 Antibody
MBS5312940-01mg	MyBiosource	0.1mg	EUR 975

ALOX5 Antibody
MBS5312940-5x01mg	MyBiosource	5x0.1mg	EUR 4245

ALOX5 Antibody
MBS2529689-006mL	MyBiosource	0.06mL	EUR 230

ALOX5 Antibody
MBS2529689-012mL	MyBiosource	0.12mL	EUR 310

ALOX5 Antibody
MBS7136531-005mL	MyBiosource	0.05mL	EUR 220

ALOX5 Antibody
MBS7136531-01mL	MyBiosource	0.1mL	EUR 300

ALOX5 Antibody
MBS7136531-5x01mL	MyBiosource	5x0.1mL	EUR 1350

ALOX5 Antibody
MBS7006095-005mg	MyBiosource	0.05mg	EUR 190

ALOX5 Antibody
MBS7006095-01mg	MyBiosource	0.1mg	EUR 270

ALOX5 Antibody
MBS7006095-5x01mg	MyBiosource	5x0.1mg	EUR 1205

ALOX5 Antibody
MBS7119279-005mg	MyBiosource	0.05mg	EUR 150

ALOX5 Antibody
MBS7119279-01mg	MyBiosource	0.1mg	EUR 190

ALOX5 Antibody
MBS7119279-5x01mg	MyBiosource	5x0.1mg	EUR 845

ALOX5 Antibody
MBS7123106-005mL	MyBiosource	0.05mL	EUR 190

ALOX5 Antibody
MBS7123106-01mL	MyBiosource	0.1mL	EUR 270

ALOX5 Antibody
MBS7123106-5x01mL	MyBiosource	5x0.1mL	EUR 1205

ALOX5 Antibody
MBS7123107-005mL	MyBiosource	0.05mL	EUR 190

ALOX5 Antibody
MBS7123107-01mL	MyBiosource	0.1mL	EUR 270

ALOX5 Antibody
MBS7123107-5x01mL	MyBiosource	5x0.1mL	EUR 1205

ALOX5 Antibody
MBS8516351-01mg	MyBiosource	0.1mg	EUR 305

ALOX5 Antibody
MBS8516351-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

ALOX5 Antibody
MBS8516351-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

ALOX5 Antibody
MBS8516351-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

ALOX5 Antibody
MBS8516351-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

ALOX5 Antibody
MBS8505300-01mL	MyBiosource	0.1mL	EUR 325

ALOX5 Antibody
MBS8505300-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

ALOX5 Antibody
MBS8505300-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

ALOX5 Antibody
MBS8505300-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

ALOX5 Antibody
MBS8505300-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

ALOX5 Antibody
MBS9404993-01mL	MyBiosource	0.1mL	EUR 305

ALOX5 Antibody
MBS9404993-5x01mL	MyBiosource	5x0.1mL	EUR 1230

ALOX5 Antibody
MBS9607534-01mL	MyBiosource	0.1mL	EUR 260

ALOX5 Antibody
MBS9607534-02mL	MyBiosource	0.2mL	EUR 305

ALOX5 Antibody
MBS9607534-5x02mL	MyBiosource	5x0.2mL	EUR 1220

ALOX5 antibody
MBS9409656-005mL	MyBiosource	0.05mL	EUR 300

ALOX5 antibody
MBS9409656-01mL	MyBiosource	0.1mL	EUR 390

ALOX5 antibody
MBS9409656-5x01mL	MyBiosource	5x0.1mL	EUR 1610

ALOX5 antibody (Ser523)
70R-14936	Fitzgerald	100 ul	EUR 497
Description: Rabbit polyclonal ALOX5 antibody (Ser523)

ALOX5 antibody (Ser271)
70R-33362	Fitzgerald	100 ug	EUR 294
Description: Rabbit polyclonal ALOX5 antibody (Ser271)

ALOX5 antibody (Ser523)
70R-34631	Fitzgerald	100 ug	EUR 294
Description: Purified Rabbit polyclonal ALOX5 antibody (Ser523)

ALOX5 antibody (Ser523)
MBS835508-01mL	MyBiosource	0.1mL	EUR 800

ALOX5 antibody (Ser523)
MBS835508-5x01mL	MyBiosource	5x0.1mL	EUR 3440

ALOX5 (pS523) Antibody
abx012014-100ug	Abbexa	100 ug	EUR 526.8

ALOX5 (pS271) Antibody
abx123322-100l	Abbexa	100 µl	EUR 200

ALOX5 (pS271) Antibody
20-abx123322	Abbexa	Ask for price Ask for price Ask for price Ask for price	20 ul 50 ul 100 ul 200 ul

ALOX5 (pS271) Antibody
abx123322-1ml	Abbexa	1 ml	EUR 475

ALOX5 (pS271) Antibody
abx123322-200l	Abbexa	200 µl	EUR 325

ALOX5 (pS663) Antibody
abx148138-100ug	Abbexa	100 ug	EUR 526.8

ALOX5 Polyclonal Antibody
A71901	EpiGentek	Ask for price Ask for price	50 ul 100 ul

ALOX5 Polyclonal Antibody
A58062	EpiGentek	Ask for price Ask for price Ask for price Ask for price	100 µg 20 ul 50 ul 100 ul

ALOX5 Conjugated Antibody
C35564	SAB	100ul	EUR 476.4

ALOX5 Polyclonal Antibody
E-AB-10040-120uL	Elabscience Biotech	120uL	EUR 240
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-10040-200uL	Elabscience Biotech	200uL	EUR 399
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-10040-20uL	Elabscience Biotech	20uL	EUR 73
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-10040-60uL	Elabscience Biotech	60uL	EUR 143
Description: Unconjugated

ALOX5 polyclonal antibody
BS6913	Bioworld Biotech	50ul	EUR 358
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

ALOX5 Polyclonal Antibody
E-AB-67417-120uL	Elabscience Biotech	120uL	EUR 320
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-67417-200uL	Elabscience Biotech	200uL	EUR 530
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-67417-60uL	Elabscience Biotech	60uL	EUR 200
Description: Unconjugated

ALOX5 Polyclonal Antibody
E-AB-67417-each	Elabscience Biotech	each	Ask for price
Description: Unconjugated

ALOX5 Polyclonal Antibody
MBS128009-002mL	MyBiosource	0.02mL	EUR 200

ALOX5 Polyclonal Antibody
MBS128009-005mL	MyBiosource	0.05mL	EUR 255

ALOX5 Polyclonal Antibody
MBS128009-01mL	MyBiosource	0.1mL	EUR 345

ALOX5 Polyclonal Antibody
MBS128009-02mL	MyBiosource	0.2mL	EUR 545

ALOX5 Polyclonal Antibody
MBS128009-5x02mL	MyBiosource	5x0.2mL	EUR 2265

ALOX5 Polyclonal Antibody
MBS2522401-002mL	MyBiosource	0.02mL	EUR 140

ALOX5 Polyclonal Antibody
MBS2522401-006mL	MyBiosource	0.06mL	EUR 180

ALOX5 Polyclonal Antibody
MBS2522401-012mL	MyBiosource	0.12mL	EUR 260

ALOX5 Polyclonal Antibody
MBS2522401-02mL	MyBiosource	0.2mL	EUR 405

ALOX5 Polyclonal Antibody
MBS2524895-002mL	MyBiosource	0.02mL	EUR 135

ALOX5 Polyclonal Antibody
MBS2524895-006mL	MyBiosource	0.06mL	EUR 190

ALOX5 Polyclonal Antibody
MBS2524895-012mL	MyBiosource	0.12mL	EUR 265

ALOX5 Polyclonal Antibody
MBS2524895-02mL	MyBiosource	0.2mL	EUR 415

ALOX5 Polyclonal Antibody
MBS2524895-5x02mL	MyBiosource	5x0.2mL	EUR 1835

ALOX5 Polyclonal Antibody
MBS2566249-006mL	MyBiosource	0.06mL	EUR 190

ALOX5 Polyclonal Antibody
MBS2566249-012mL	MyBiosource	0.12mL	EUR 265

ALOX5 Polyclonal Antibody
MBS2566249-02mL	MyBiosource	0.2mL	EUR 415

ALOX5 Polyclonal Antibody
MBS2566249-5x02mL	MyBiosource	5x0.2mL	EUR 1835

ALOX5 Conjugated Antibody
MBS9446036-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

ALOX5 Conjugated Antibody
MBS9446036-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

ALOX5 Conjugated Antibody
MBS9446036-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

ALOX5 Conjugated Antibody
MBS9446036-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

ALOX5 Conjugated Antibody
MBS9446036-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

ALOX5 Polyclonal Antibody
RD75005A-120uL	Reddot Biotech	120μL	EUR 360
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD75005A-200uL	Reddot Biotech	200μL	EUR 630
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD75005A-20uL	Reddot Biotech	20μL	EUR 109.5
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD75005A-60uL	Reddot Biotech	60μL	EUR 214.5
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD267417A-120uL	Reddot Biotech	120μL	EUR 360
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD267417A-200uL	Reddot Biotech	200μL	EUR 630
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Polyclonal Antibody
RD267417A-60uL	Reddot Biotech	60μL	EUR 204
Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ALOX5 Antibody - middle region
MBS3222309-01mL	MyBiosource	0.1mL	EUR 455

ALOX5 Antibody - middle region
MBS3222309-5x01mL	MyBiosource	5x0.1mL	EUR 1995

ALOX5 Antibody, HRP conjugated
1-CSB-PA001624LB01HU	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ALOX5 Antibody, HRP conjugated
MBS1499651-005mg	MyBiosource	0.05mg	EUR 190

ALOX5 Antibody, HRP conjugated
MBS1499651-01mg	MyBiosource	0.1mg	EUR 270

ALOX5 Antibody, HRP conjugated
MBS1499651-5x01mg	MyBiosource	5x0.1mg	EUR 1205

ALOX5 Antibody, FITC conjugated
1-CSB-PA001624LC01HU	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ALOX5 Antibody, FITC conjugated
MBS1499096-005mg	MyBiosource	0.05mg	EUR 190

ALOX5 Antibody, FITC conjugated
MBS1499096-01mg	MyBiosource	0.1mg	EUR 270

ALOX5 Antibody, FITC conjugated
MBS1499096-5x01mg	MyBiosource	5x0.1mg	EUR 1205

OACA02570-50UG - ALOX5 Antibody
OACA02570-50UG	Aviva Systems Biology	50ug	EUR 179

ALOX5 Antibody, Biotin conjugated
1-CSB-PA001624LD01HU	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against ALOX5. Recognizes ALOX5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ALOX5 Antibody, Biotin conjugated
MBS1493680-005mg	MyBiosource	0.05mg	EUR 190

ALOX5 Antibody, Biotin conjugated
MBS1493680-01mg	MyBiosource	0.1mg	EUR 270

ALOX5 Antibody, Biotin conjugated
MBS1493680-5x01mg	MyBiosource	5x0.1mg	EUR 1205

OAEB01837-100UG - ALOX5 Antibody
OAEB01837-100UG	Aviva Systems Biology	100ug	EUR 349

OACA02570-100UG - ALOX5 Antibody
OACA02570-100UG	Aviva Systems Biology	100ug	EUR 319

ALOX5 (Phospho- Ser663) Antibody
ABF8359	Lifescience Market	100 ug	EUR 525.6

Phospho- ALOX5 (Ser523) Antibody
ABF0064	Lifescience Market	100 ug	EUR 525.6

ALOX5 (E267) polyclonal antibody
BS1506	Bioworld Biotech	50ul	EUR 275
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

ALOX5 (Phospho-Ser663) Antibody
12671-100ul	SAB	100ul	EUR 302.4

ALOX5 (Phospho-Ser663) Antibody
12671-50ul	SAB	50ul	EUR 224.4

ALOX5 (Phospho-Ser523) Antibody
12128	SAB	100ul	EUR 319

ALOX5 (Phospho-Ser523) Antibody
12128-100ul	SAB	100ul	EUR 302.4

ALOX5 (Phospho-Ser523) Antibody
12128-50ul	SAB	50ul	EUR 224.4

ALOX5 (Phospho-Ser523) Antibody
E012128	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

ALOX5 (Phospho-Ser523) Antibody
E11-8487A	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

ALOX5 (Phospho-Ser523) Antibody
MBS9404495-005mL	MyBiosource	0.05mL	EUR 245

ALOX5 (Phospho-Ser523) Antibody
MBS9404495-01mL	MyBiosource	0.1mL	EUR 305

ALOX5 (Phospho-Ser523) Antibody
MBS9404495-5x01mL	MyBiosource	5x0.1mL	EUR 1230

ALOX5 (Phospho-Ser523) Antibody
MBS858202-01mg	MyBiosource	0.1mg	EUR 305

ALOX5 (Phospho-Ser523) Antibody
MBS858202-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

ALOX5 (Phospho-Ser523) Antibody
MBS858202-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

ALOX5 (Phospho-Ser523) Antibody
MBS858202-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

ALOX5 (Phospho-Ser523) Antibody
MBS858202-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465